Thread the rotor lid onto the rotor spindle and tighten it securely clockwise. Never operate the centrifuge without the rotor lid securely fastened. Running the Centrifuge
Maintain a minimum clearance zone of 30 cm (12 inches) on all sides of the unit. This space ensures adequate ventilation for the cooling fan and complies with laboratory safety regulations in the event of a rotor failure.
Eppendorf maintains a support portal. Navigate to eppendorf.com > Support > Downloads . Search for "5402." Note that Eppendorf often classifies the 5402 under "Historical Products." You may need to register a free account to access legacy manuals.
Use the manual emergency lid release string/slot located under the front base of the unit. 7. Professional Service and Calibration eppendorf 5402 manual
Remove the rotor regularly to clean the shaft and the underside of the rotor.
Press down firmly on the lid. Check the main fuse and power cord. Rotor imbalance or loose rotor lid.
The Eppendorf 5402 is a discontinued model. If you contact Eppendorf directly, they are likely to direct you to its successors: the 5418 R or 5425 R refrigerated centrifuges. Thread the rotor lid onto the rotor spindle
is a refrigerated centrifuge designed for rapid separation of laboratory samples. Its primary application involves spinning microcentrifuge tubes (1.5 mL to 2.0 mL) at high speeds while maintaining low temperatures, crucial for maintaining sample integrity in DNA/RNA isolation, protein purification, and enzymatic reactions. Refrigeration: Temperature range from
Refrigerated runs generate moisture inside the aluminum chamber. Leave the main lid open after your work shift to let the chamber dry out, preventing rust and mold.
If you need specific troubleshooting for an error code or need guidance on replacing the motor brushes, let me know! This space ensures adequate ventilation for the cooling
Controls the refrigeration system. For sensitive sample preparation, it is recommended to set this dial 10 to 15 minutes before running samples to allow the chamber to pre-cool.
Maintain at least 30 cm (12 inches) of open space on all sides of the unit to ensure adequate ventilation for the cooling fan and refrigeration unit.
, a classic refrigerated microcentrifuge designed for laboratory research. Eppendorf Centrifuge 5402 : Technical Overview and Operational Guide The Eppendorf 5402
If the centrifuge malfunctions, check these common causes before contacting technical support. Potential Cause Recommended Action Main power disconnected or blown fuse.
: Modern centrifugation relies on the strict maintenance of center-of-mass alignment. The paper discusses the physics of rotor imbalance—where even a 0.1g variance at 14,000 RPM translates into significant destructive torque. The RCF Equation
